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Registros recuperados: 7
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Addition of proteic nitrogen during alcoholic fermentation for the production of cachaça Scientia Agricola
Jeronimo,Elisangela Marques; Oliveira,Evelyn de Souza; Souza,Elson Luíz Rocha; Silva,Marcelo de Almeida; Serra,Gil Eduardo.
Cachaça is the denomination of a typical and exclusive Brazilian spirit produced from the distillation of fermented sugarcane juice must. The objective of this study was to evaluate the effect of adding yeast extract to the sugarcane juice used for sugarcane liquor production, because for the artisanal process no studies are available on nitrogen addition nor beverage quality, involving nitrogen complementation. Results of previous studies in the laboratory scale showed that sugarcane juice complementation with proteic nitrogen can be a beneficial practice for yeast multiplication and cellular growth, and also for the improvement of fermentation yield and liquor productivity. In this pilot scale study, using recycled yeast, the addition of proteic nitrogen...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Yeast extract; Cell viability; Volatile compounds; Sensory analysis.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162008000200009
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Comparative growth of trichoderma strains in different nutritional sources, using bioscreen c automated system BJM
Rossi-Rodrigues,Bianca Caroline; Brochetto-Braga,Márcia Regina; Tauk-Tornisielo,Sâmia Maria; Carmona,Eleonora Cano; Arruda,Valeska Marques; Chaud Netto,José.
Trichoderma is one of the fungi genera that produce important metabolites for industry. The growth of these organisms is a consequence of the nutritional sources used as also of the physical conditions employed to cultivate them. In this work, the automated Bioscreen C system was used to evaluate the influence of different nutritional sources on the growth of Trichoderma strains (T. hamatum, T. harzianum, T. viride, and T. longibrachiatum) isolated from the soil in the Juréia-Itatins Ecological Station (JIES), São Paulo State - Brazil.The cultures were grown in liquid culture media containing different carbon- (2%; w/v) and nitrogen (1%; w/v) sources at 28ºC, pH 6.5, and agitated at 150 rpm for 72 h. The results showed, as expected, that glucose is...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bioscreen C system; Growth; Trichoderma; Tryptone; Yeast extract.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200035
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Ethanol induction of laccase depends on nitrogen conditions of Pycnoporus sanguineus Electron. J. Biotechnol.
Hernández,Christian A; Sandoval,Norma; Mallerman,Julieta; García-Pérez,José Antonio; Farnet,Anne-Marie; Perraud-Gaime,Isabelle; Alarcón,Enrique.
Background Ethanol has been pointed out as a laccase inducer. However, there are controversial reports about its efficiency with some fungi. In this study, we hypothesized that ethanol laccase induced in Pycnoporus sanguineus depends on nitrogen nutriment conditions. To prove this, we assessed laccase production in submerged cultures of P. sanguineus, with different nitrogen concentrations and with, or without ethanol added in a factorial designed experiment. Results In order to analyze the effects of factors on the response variables, a factorial ANOVA, and response-surface models were performed. It was found that the nitrogen source was the main factor that affected laccase production in P. sanguineus. The treatments with yeast extract (2 g/L) and...
Tipo: Journal article Palavras-chave: Enzyme production; Fungal metabolism; Phenoloxidases; Urea; Yeast extract.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000400012
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Improvement in RNA extraction from S. cerevisie by optimization in the autolysis and NH3 hydrolysis BABT
Oliveira,Antonio Martins; Oliva Neto,Pedro de.
The optimization of autolysis of Saccharomyces cerevisiae from brewery was studied aiming at the maximum ribonucleic acid extraction and yeast extract production. The best conditions for yeast autolysis was 55.2ºC, pH= 5.1 and 9.8% NaCl for 24h of processing, without the NH3 use. In these conditions, the RNA yield was 89.7%, resulting in 51.3% of dehydrated yeast extract with 57.9% protein. The use of 12.2% NH3 at 60ºC after autolysis (8h) and plasmolysis (8h) was not viable due to the reduction in the RNA yield from 89.7to78.4%. On the other hand, the thermal shock at 60ºC for 15 minutes prior to autolysis provided an increase in the yield from 89.7 to 91.4%. The autolysis, including NaCl plasmolysis in the optimized conditions was efficient, economic and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Autolysis; NaCl plasmolysis; Yeast extract; RNA extraction; Nucleotides.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000500019
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Optimization of asparaginase production from Zymomonas mobilis by continuous fermentation Biological Sciences
Menegat, Francieli Bortoluzzi; Baldo, Cristiani; Celligoi, Maria Antonia Pedrine Colabone; Buzato, João Batista.
Asparaginase is an enzyme used in clinical treatments as a chemotherapeutic agent and in food technology to prevent acrylamide formation in fried and baked foods. Asparaginase is industrially produced by microorganisms, mainly gram-negative bacteria. Zymomonas mobilis is a Gram-negative bacterium that utilizes glucose, fructose and sucrose as carbon source and has been known for its efficiency in producing ethanol, sorbitol, levan, gluconic acid and has recently aroused interest for asparaginase production. Current assay optimizes the production of Z. mobilis asparaginase by continuous fermentation using response surface experimental design and methodology. The studied variables comprised sucrose, yeast extract and asparagine. Optimized condition obtained...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sucrose; Asparagine; Yeast extract; Enzyme; Microbial production; Factorial design.
Ano: 2016 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/31057
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Production of yeast extract from whey using Kluyveromyces marxianus BABT
Revillion,Jean P. de Palma; Brandelli,Adriano; Ayub,Marco A. Záchia.
The yeast Kluyveromyces marxianus CBS 6556 was grown on whey to produce nucleotide-rich yeast extracts. Thermal treatments of cells at 35 or 50ºC for 15-30h resulted in yeast extracts containing about 20 g/L protein, with only the second treatment resulting in the presence of small amounts of RNA. In contrast, autolysis in buffered solution was the unique treatment that resulted in release of high amounts of intracellular RNA, being, therefore, the better procedure to produce 5'-nucletide rich extract with K. marxianus.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cheese whey; Yeast extract; 5'-nucleotide; Food flavour enhancer.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000100017
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Production of yellow-green fluorescent pigment by Pseudomonas fluorescens BABT
Silva,Gildo Almeida da; Almeida,Erik Amazonas de.
A medium was prepared from brewery waste yeast with and without mineral salts to study growth and yellow-green fluorescent pigment production (YGFP) by Pseudomonas fluorescens. The King's medium used for detection of siderophore production were expressively weaker inductors of YGFP formation when compared to FYE medium. Although FYE and CYE could be used for growth of P. fluorescens, only FYE was an attractive medium for detection of YGFP strain producers.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pseudomonas fluorescens; Siderophore; Yeast extract; Yellow-green fluorescent pigment.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000400009
Registros recuperados: 7
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